Safety assessment of a newly synthesized copolymer for micellar delivery of hydrophobic caffeic acid phenethyl ester.
Identifieur interne : 000135 ( Main/Exploration ); précédent : 000134; suivant : 000136Safety assessment of a newly synthesized copolymer for micellar delivery of hydrophobic caffeic acid phenethyl ester.
Auteurs : Yordan Yordanov [Bulgarie] ; Denitsa Aluani [Bulgarie] ; Virginia Tzankova [Bulgarie] ; Stanislav Rangelov [Bulgarie] ; Feodor Odzhakov [Bulgarie] ; Alexandar Apostolov [Bulgarie] ; Krassimira Yoncheva [Bulgarie]Source :
- Pharmaceutical development and technology [ 1097-9867 ] ; 2020.
Abstract
Caffeic acid phenethyl ester (CAPE), a major pharmacologically active component of poplar type propolis, is known for its proapoptotic, anti-inflammatory, antioxidant, antiviral, and enzyme inhibiting activities. The aim of this study was to perform an in vitro and in vivo safety assessment of a micellar system based on a newly synthesized copolymer, consisting of polyglycidol and poly(allyl glycidyl ether) (C12-PAGE-PG) as a drug delivery platform for CAPE. The in vitro studies on HepG2 and L929 cells by MTT and LDH assays after treatment with the empty and CAPE-loaded micelles showed no cytotoxic effects of the empty micelles and retained cytotoxic activity of CAPE loaded in the micelles. No hemolysis or stimulation of mouse lymphocytes or macrophages was observed in vitro. In vivo hematological, biochemical, and histological assays on rats, treated with the empty (2580 and 5160 µg/kg) or CAPE-loaded (375 and 750 µg CAPE/kg) micelles did not reveal pathological changes of any of the parameters assayed after 14-days' treatment. In conclusion, initial toxicological data characterize C12-PAGE-PG as a non-toxic and promising copolymer for development of micellar drug delivery systems, particularly for a hydrophobic active substance as CAPE.
DOI: 10.1080/10837450.2020.1818259
PubMed: 32892659
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Safety assessment of a newly synthesized copolymer for micellar delivery of hydrophobic caffeic acid phenethyl ester.</title><author><name sortKey="Yordanov, Yordan" sort="Yordanov, Yordan" uniqKey="Yordanov Y" first="Yordan" last="Yordanov">Yordan Yordanov</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Aluani, Denitsa" sort="Aluani, Denitsa" uniqKey="Aluani D" first="Denitsa" last="Aluani">Denitsa Aluani</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Tzankova, Virginia" sort="Tzankova, Virginia" uniqKey="Tzankova V" first="Virginia" last="Tzankova">Virginia Tzankova</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Rangelov, Stanislav" sort="Rangelov, Stanislav" uniqKey="Rangelov S" first="Stanislav" last="Rangelov">Stanislav Rangelov</name><affiliation wicri:level="3"><nlm:affiliation>Bulgarian Academy of Sciences, Institute of Polymers, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Bulgarian Academy of Sciences, Institute of Polymers, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Odzhakov, Feodor" sort="Odzhakov, Feodor" uniqKey="Odzhakov F" first="Feodor" last="Odzhakov">Feodor Odzhakov</name><affiliation wicri:level="3"><nlm:affiliation>Department of Forensic Medicine and Deontology, Medical Faculty, Medical University of Sofia, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Forensic Medicine and Deontology, Medical Faculty, Medical University of Sofia, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Apostolov, Alexandar" sort="Apostolov, Alexandar" uniqKey="Apostolov A" first="Alexandar" last="Apostolov">Alexandar Apostolov</name><affiliation wicri:level="3"><nlm:affiliation>Department of Forensic Medicine and Deontology, Medical Faculty, Medical University of Sofia, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Forensic Medicine and Deontology, Medical Faculty, Medical University of Sofia, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Yoncheva, Krassimira" sort="Yoncheva, Krassimira" uniqKey="Yoncheva K" first="Krassimira" last="Yoncheva">Krassimira Yoncheva</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Medical University of Sofia, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32892659</idno><idno type="pmid">32892659</idno><idno type="doi">10.1080/10837450.2020.1818259</idno><idno type="wicri:Area/Main/Corpus">000117</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000117</idno><idno type="wicri:Area/Main/Curation">000117</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000117</idno><idno type="wicri:Area/Main/Exploration">000117</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Safety assessment of a newly synthesized copolymer for micellar delivery of hydrophobic caffeic acid phenethyl ester.</title><author><name sortKey="Yordanov, Yordan" sort="Yordanov, Yordan" uniqKey="Yordanov Y" first="Yordan" last="Yordanov">Yordan Yordanov</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Aluani, Denitsa" sort="Aluani, Denitsa" uniqKey="Aluani D" first="Denitsa" last="Aluani">Denitsa Aluani</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Tzankova, Virginia" sort="Tzankova, Virginia" uniqKey="Tzankova V" first="Virginia" last="Tzankova">Virginia Tzankova</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Rangelov, Stanislav" sort="Rangelov, Stanislav" uniqKey="Rangelov S" first="Stanislav" last="Rangelov">Stanislav Rangelov</name><affiliation wicri:level="3"><nlm:affiliation>Bulgarian Academy of Sciences, Institute of Polymers, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Bulgarian Academy of Sciences, Institute of Polymers, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Odzhakov, Feodor" sort="Odzhakov, Feodor" uniqKey="Odzhakov F" first="Feodor" last="Odzhakov">Feodor Odzhakov</name><affiliation wicri:level="3"><nlm:affiliation>Department of Forensic Medicine and Deontology, Medical Faculty, Medical University of Sofia, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Forensic Medicine and Deontology, Medical Faculty, Medical University of Sofia, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Apostolov, Alexandar" sort="Apostolov, Alexandar" uniqKey="Apostolov A" first="Alexandar" last="Apostolov">Alexandar Apostolov</name><affiliation wicri:level="3"><nlm:affiliation>Department of Forensic Medicine and Deontology, Medical Faculty, Medical University of Sofia, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Forensic Medicine and Deontology, Medical Faculty, Medical University of Sofia, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Yoncheva, Krassimira" sort="Yoncheva, Krassimira" uniqKey="Yoncheva K" first="Krassimira" last="Yoncheva">Krassimira Yoncheva</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Medical University of Sofia, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author></analytic><series><title level="j">Pharmaceutical development and technology</title><idno type="eISSN">1097-9867</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Caffeic acid phenethyl ester (CAPE), a major pharmacologically active component of poplar type propolis, is known for its proapoptotic, anti-inflammatory, antioxidant, antiviral, and enzyme inhibiting activities. The aim of this study was to perform an <i>in vitro</i> and <i>in vivo</i> safety assessment of a micellar system based on a newly synthesized copolymer, consisting of polyglycidol and poly(allyl glycidyl ether) (C<sub>12</sub>-PAGE-PG) as a drug delivery platform for CAPE. The <i>in vitro</i> studies on HepG2 and L929 cells by MTT and LDH assays after treatment with the empty and CAPE-loaded micelles showed no cytotoxic effects of the empty micelles and retained cytotoxic activity of CAPE loaded in the micelles. No hemolysis or stimulation of mouse lymphocytes or macrophages was observed <i>in vitro</i>. <i>In vivo</i> hematological, biochemical, and histological assays on rats, treated with the empty (2580 and 5160 µg/kg) or CAPE-loaded (375 and 750 µg CAPE/kg) micelles did not reveal pathological changes of any of the parameters assayed after 14-days' treatment. In conclusion, initial toxicological data characterize C<sub>12</sub>-PAGE-PG as a non-toxic and promising copolymer for development of micellar drug delivery systems, particularly for a hydrophobic active substance as CAPE.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32892659</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1097-9867</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>10</Issue><PubDate><Year>2020</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Pharmaceutical development and technology</Title><ISOAbbreviation>Pharm Dev Technol</ISOAbbreviation></Journal><ArticleTitle>Safety assessment of a newly synthesized copolymer for micellar delivery of hydrophobic caffeic acid phenethyl ester.</ArticleTitle><Pagination><MedlinePgn>1271-1280</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/10837450.2020.1818259</ELocationID><Abstract><AbstractText>Caffeic acid phenethyl ester (CAPE), a major pharmacologically active component of poplar type propolis, is known for its proapoptotic, anti-inflammatory, antioxidant, antiviral, and enzyme inhibiting activities. The aim of this study was to perform an <i>in vitro</i> and <i>in vivo</i> safety assessment of a micellar system based on a newly synthesized copolymer, consisting of polyglycidol and poly(allyl glycidyl ether) (C<sub>12</sub>-PAGE-PG) as a drug delivery platform for CAPE. The <i>in vitro</i> studies on HepG2 and L929 cells by MTT and LDH assays after treatment with the empty and CAPE-loaded micelles showed no cytotoxic effects of the empty micelles and retained cytotoxic activity of CAPE loaded in the micelles. No hemolysis or stimulation of mouse lymphocytes or macrophages was observed <i>in vitro</i>. <i>In vivo</i> hematological, biochemical, and histological assays on rats, treated with the empty (2580 and 5160 µg/kg) or CAPE-loaded (375 and 750 µg CAPE/kg) micelles did not reveal pathological changes of any of the parameters assayed after 14-days' treatment. In conclusion, initial toxicological data characterize C<sub>12</sub>-PAGE-PG as a non-toxic and promising copolymer for development of micellar drug delivery systems, particularly for a hydrophobic active substance as CAPE.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yordanov</LastName><ForeName>Yordan</ForeName><Initials>Y</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-1029-9870</Identifier><AffiliationInfo><Affiliation>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aluani</LastName><ForeName>Denitsa</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tzankova</LastName><ForeName>Virginia</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rangelov</LastName><ForeName>Stanislav</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Bulgarian Academy of Sciences, Institute of Polymers, Sofia, Bulgaria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Odzhakov</LastName><ForeName>Feodor</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Forensic Medicine and Deontology, Medical Faculty, Medical University of Sofia, Sofia, Bulgaria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Apostolov</LastName><ForeName>Alexandar</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Forensic Medicine and Deontology, Medical Faculty, Medical University of Sofia, Sofia, Bulgaria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yoncheva</LastName><ForeName>Krassimira</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Pharm Dev Technol</MedlineTA><NlmUniqueID>9610932</NlmUniqueID><ISSNLinking>1083-7450</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">CAPE-loaded micelles</Keyword><Keyword MajorTopicYN="N">Honeybee propolis</Keyword><Keyword MajorTopicYN="N">biocompatibility</Keyword><Keyword MajorTopicYN="N">polyglycidol copolymer</Keyword><Keyword MajorTopicYN="N">toxicology</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>7</Day><Hour>5</Hour><Minute>25</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32892659</ArticleId><ArticleId IdType="doi">10.1080/10837450.2020.1818259</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Bulgarie</li></country><region><li>Sofia-ville (oblast)</li></region><settlement><li>Sofia</li></settlement></list><tree><country name="Bulgarie"><region name="Sofia-ville (oblast)"><name sortKey="Yordanov, Yordan" sort="Yordanov, Yordan" uniqKey="Yordanov Y" first="Yordan" last="Yordanov">Yordan Yordanov</name></region><name sortKey="Aluani, Denitsa" sort="Aluani, Denitsa" uniqKey="Aluani D" first="Denitsa" last="Aluani">Denitsa Aluani</name><name sortKey="Apostolov, Alexandar" sort="Apostolov, Alexandar" uniqKey="Apostolov A" first="Alexandar" last="Apostolov">Alexandar Apostolov</name><name sortKey="Odzhakov, Feodor" sort="Odzhakov, Feodor" uniqKey="Odzhakov F" first="Feodor" last="Odzhakov">Feodor Odzhakov</name><name sortKey="Rangelov, Stanislav" sort="Rangelov, Stanislav" uniqKey="Rangelov S" first="Stanislav" last="Rangelov">Stanislav Rangelov</name><name sortKey="Tzankova, Virginia" sort="Tzankova, Virginia" uniqKey="Tzankova V" first="Virginia" last="Tzankova">Virginia Tzankova</name><name sortKey="Yoncheva, Krassimira" sort="Yoncheva, Krassimira" uniqKey="Yoncheva K" first="Krassimira" last="Yoncheva">Krassimira Yoncheva</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000135 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000135 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32892659
|texte= Safety assessment of a newly synthesized copolymer for micellar delivery of hydrophobic caffeic acid phenethyl ester.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32892659" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |